. 2019 Jun 20;9:8970. doi: 10.1038/s41598-019-45328-3

Table 1.

Successful (+) or unsuccessful (−) PCR amplifications of 8 major HIV-1 subtypes in three independent assays.

HIV-1 Subtype	Plasma VL (RNA copies/ml)	10,000	5,000	2,500	1,000	500	200	100	50
HIV-1 Subtype	*RNA copy # per RT-PCR reaction	330	165	82.5	33	16.5	6.6	3.3	1.6
A1		+++	+++	+++	+++	+++	−++	+++	−++
CRF01_AE		+++	+++	+++	+++	+++	+++	+++	−−+
CRF02_AG		+++	+++	+++	+++	+++	+−−	−−−	−−+
B		+++	+++	+++	+++	+++	+++	−−+	−−−
C		+++	+++	+++	+++	+++	−−+	+++	−−−
D		+++	+++	+++	++−	++−	−−−	−−−	−−−
F2		+++	+++	+++	+++	+++	+++	−+−	−−−
G		+++	+++	+++	+++	+++	+++	++−	++−

*Estimated viral RNA copy number in the PCR was calculated based on 90% extraction efficiency.