Table 2.

Sensitivity for the detection of minor variants in mixed plasmid populations by the MiSeq-HyDRA platform. *n/d = not detected.

Plasmid Mixture (%)		P1	M1	M2	M3	M4	M5	M6	M7	M8	M9	M10	M11	M12	M13	P2
P1 (G73T_K103N)		100	99.9	99.5	99	95	90	80	50	20	10	5	1	0.5	0.1	0
P2 (G73T_K65R)		0	0.1	0.5	1	5	10	20	50	80	90	95	99	99.5	99.9	100
Mutation	Replicate	Variant Frequency Detected (%)
G73T(P1 & P2)	1	99.62	99.59	99.61	99.65	99.64	99.65	99.68	99.57	99.62	99.60	99.61	99.59	99.61	99.56	99.50
	2	99.58	99.60	99.64	99.49	99.65	99.62	99.55	99.17	99.56	99.55	99.58	99.55	99.57	99.65	99.61
	3	99.61	99.56	99.60	99.55	99.60	99.55	99.54	99.57	99.60	99.60	99.62	99.53	99.56	99.52	99.65
	AVG	99.60	99.58	99.62	99.56	99.63	99.61	99.59	99.44	99.59	99.58	99.60	99.56	99.58	99.58	99.59
K103N (P1)	1	99.11	98.90	98.36	97.69	92.06	85.42	73.81	48.51	21.66	11.66	5.88	1.25	0.50	n/d	n/d
	2	99.34	98.92	98.59	97.71	92.29	85.91	72.95	48.06	21.42	11.50	6.03	1.15	0.60	n/d	n/d
	3	99.12	98.74	98.21	97.52	91.45	84.54	72.30	48.59	21.54	12.39	5.50	1.17	0.49	n/d	n/d
	AVG	99.19	98.85	98.39	97.64	91.93	85.29	73.02	48.39	21.54	11.85	5.80	1.19	0.53	n/d	n/d
K65R (P2)	1	n/d	0.58	1.23	2.02	7.50	14.82	25.73	49.97	74.60	84.75	92.91	98.05	98.64	99.15	99.26
	2	n/d	0.62	1.16	1.77	7.49	14.29	26.75	48.44	76.34	86.50	92.70	97.76	98.75	99.28	99.24
	3	n/d	0.64	1.13	2.04	8.58	15.10	26.79	48.85	75.90	85.43	92.72	97.95	98.79	99.15	99.74
	AVG	n/d	0.61	1.17	1.94	7.86	14.74	26.42	49.09	75.61	85.56	92.78	97.92	98.73	99.19	99.41