Table 4.
Intra-assay precision for LADRVs identified by the MiSeq-HyDRA assay.
| Sample | Gene | Class | DRM | Rep1¶ | Rep 2¶ | Rep3¶ | SS* |
|---|---|---|---|---|---|---|---|
| SDR-55 | PR | Other | K20I | 98.97 | 98.78 | 99.06 | YES |
| PR | Other | V82I | 99.25 | 99.24 | 99.21 | YES | |
| RT | Other | V90I | 2.83 | ND | ND | NO | |
| RT | Other | V118I | ND | 9.88 | ND | NO | |
| RT | Other | V179I | 1.44 | 7.98 | 1.38 | NO | |
| RT | NRTI | M184V | 99.34 | 99.08 | 99.19 | YES | |
| RT | Other | L210S | 2.44 | ND | ND | NO | |
| SDR-95 | PR | Other | V11I | 94.58 | 96.59 | 95.85 | YES |
| PR | Other | K20I | 99.31 | 97.07 | 99.18 | YES | |
| PR | Accessory | K20T | ND | 2.2 | ND | NO | |
| PR | Accessory | L33F | 17.54 | 29.3 | 17.88 | YES | |
| PR | Major | M46I | ND | 1.47 | ND | NO | |
| PR | Other | V82I | 42.21 | 44.48 | 35.9 | YES | |
| RT | NRTI | K65E | ND | ND | 1.86 | NO | |
| RT | Other | K101R | 10.68 | 9.04 | 8.38 | YES | |
| RT | Other | V106I | 1.55 | ND | ND | NO | |
| RT | NNRTI | V108I | ND | 1.04 | ND | NO | |
| SDR-140 | PR | Other | L10I | 98.69 | 98.63 | 98.74 | YES |
| PR | Accessory | N88D | ND | 6.89 | ND | NO | |
| RT | NRTI | L74V | ND | 1.13 | ND | NO | |
| RT | NNRTI | K103N | 99.41 | 99.08 | 99.41 | YES | |
| RT | NNRTI | P225H | 98.95 | 83.73 | 99.12 | YES | |
| SDR-143 | RT | NRTI | A62V | ND | 3.11 | ND | NO |
| RT | NNRTI | K103N | 99.3 | 99.39 | 99.35 | YES |
SDRMs are highlighted in bold. ¶Mutation frequencies (%) detected in replicates; *Detection by Sanger-based sequencing assay.