Fig. 2.

Targeted mutagenesis of OsIAA23 in rice using CRISPR/Cas system. a Diagram of gene structure and conserved domains of OsIAA23 (Os06t0597000-01 for representation). The black rectangles represent the exon regions. The short lines are marked with Roman numerals bellow indicate four domains of OsIAA23, and the spacer location is marked by red. b sgRNA:Cas9-induced rice OsIAA23 gene mutations at the target site in the mutants. The sequence marked in the red text under the black line indicates the spacer sequence. c Amino acid sequence alignment of wild type OsIAA23 protein and the Osiaa23 mutants. The sequence marked in the red text indicated the spacer location, and Domain II is indicated by underlined text