Table 6.

Hierarchical ANOVA and post hoc Bonferroni comparisons performed on hs-TnT, fibrinogen, TNF-α, LPS, and on hs-CRP, IFN-γ, IL-1β, IL-6, IL-8 after logarithmic transformation, and IL-12 after square root transformation, in patients with and without hs-TnT rise

Biomarker	P-values between-subject effects using hierarchical ANOVAa			P-values between different time points using post hoc Bonferronib
	Groupc	Timed	Group × timee	Pre–Postf	Pre-D1g	Post-D1h
• Biomarkers of systemic inflammation
hs-CRP log	0.139	<0.001	0.994	>0.999	<0.001	<0.001
Fibrinogen	0.352	0.012	0.812	>0.999	0.037	0.005
IFN-γ log	0.667	<0.001	0.176	>0.999	<0.001	<0.001
TNF-α	0.434	0.096	0.138	>0.999	0.123	0.034
IL-6 log	0.697	<0.001	0.811	>0.999	<0.001	<0.001
IL-8 log	0.404	<0.001	0.652	0.028	<0.001	0.011
IL-12 sqrt	0.434	<0.001	0.218	>0.999	<0.001	<0.001
• Biomarker of endotoxemia (LPS endotoxin)
LPS(LAL)	0.888	<0.001	0.356	0.647	<0.001	<0.001

IL interleukin, IFN-γ interferon-γ, hs-TnT highly sensitive troponin T, hs-CRP highly sensitive C-reactive protein, LPS lipopolysaccharide, TNF-α tumor necrosis factor-α

^aHierarchical ANOVA at a significance level of 0.025 performed on biomarkers that were found significant at the 0.05 level by paired t-test after logarithmic or square root transformation

^bPost hoc Bonferroni comparisons made to compare mean values at different time points of the ANOVA

at a significance level of 0.025 (significant difference shown in red)

^cGroup = comparing hs-TnT rise vs. non-rise patients

^dTime = comparing the different time points (pretreatment, immediately post treatment, and day-1 treatment)

^eGroup × time = comparing the interaction between the groups and time points

^fPre–Post = comparing pretreatment and immediately post treatment

^gPre-D1 = comparing pretreatment and day-1 treatment

^hPost-D1 = comparing immediately post treatment and day-1 treatment