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. 2019 Jun 20;9:8857. doi: 10.1038/s41598-019-45410-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Generation of transgenic flies for the expression of the Dapma-cryA-D. (A–D) Flowchart of the UAS-Dapma-crys generation process. (A) The sequences of four cry genes in the D. magna genome were identified. (B) Each cry gene was cloned and inserted into the pUAST-attB vector. The myc-tag was placed at the N-terminal end of each cry cDNA. (C) Each construct was injected into the attP-containing embryos that express Phic31. Phic31 is an integrase that mediates the site-specific recombination at the attP site, eliminating variation in insertion sites. (D) Each transformant carried a myc-Dapma-cry downstream of the UAS sequence.