Figure 5.
Re-entrainment shift after an 8-h LD delay. (A) Determination of the speed of re-entrainment between the evening activity peak of the 8-h LD phase shift. The phase of the evening activity peak was determined on the day when the LD cycle was shifted. The next day was regarded as day 1, when the activity phase shift occurred. (B) The speed of entrainment shifts on day 1 (left) and day 2 (right) after an 8 h LD phase delay. Tim-Drome-CRY (tim62-gal4/UAS-Drome-cry; cry01/cry01, n = 30) flies were phase shifted by 7 h in response to an 8 h LD shift on the first day, suggesting that these flies had almost achieved re-entrainment within one day. However, control (tim62-Gal4/+; cry01/cry01, n = 29) and each Tim-Dapma-CRY (tim62-gal4/UAS-Dapma-cryA; cry01/cry01, n = 30; tim62-gal4/UAS-Dapma-cryB; cry01/cry01, n = 30; tim62-gal4/UAS-Dapma-cryC; cry01/cry01, n = 30; tim62-gal4/UAS-Dapma-cryD; cry01/cry01, n = 30) were phase-shifted by 2 h in response to an 8-h LD shift.