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. 2019 Jun 20;9:8853. doi: 10.1038/s41598-019-45356-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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T7 Exo is inhibited by 20-nt 5′ overhangs. (a) 12 nM T7 Exo-mediated resection on 50 nM substrates presenting a blunt (red), 2- (orange), 10- (green) and 20-nt (purple) 5′ overhang with. The negative control was synthesised with two 5′-BITEG modifications (blue). Results were normalised against their respective negative controls and converted to bp. (b) Analysis of the rate of resection on the substrates in (a). Error bars represent SEM; n = 3 in all cases; **p < 0.01, ****p < 0.0001.