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. 2019 Jun 20;9:8853. doi: 10.1038/s41598-019-45356-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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ExoIII and T7 Exo resect through single-nucleotide mismatches. (a) 5 nM and (b) 10 nM ExoIII was added to perfectly matched substrates (_*S and S^*) and substrates containing four T:G or A:C mismatches, (_*S^C>T and _G>AS^*, respectively), and the resection reaction followed. Standard curve is represented by the grey dotted lines. (c) Calculated resection rate of ExoIII on the complementary and mismatched substrates based on maximum gradients in (a) and (b). (d) Time (seconds) until the ExoIII reaction reaches completion based on the point at which the reaction plateaus. (e) 2 nM, (f) 7.5 nM and (g) 10 nM T7 Exo was added to complementary substrates (S_* and ^*S) and substrates containing four T:G or A:C mismatches (^C>TS_* and ^*S_G>A, respectively). Standard curve is represented by the grey dotted lines. (h) Calculated resection rate of T7 Exo on the complementary and mismatched substrates. Error bars represent SEM; n = 3 in all cases; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.