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. 2019 Jun 20;9:8853. doi: 10.1038/s41598-019-45356-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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QI fluorescent-based toolkit reiterates RNase A and RNase H activities ssRNA and DNA/RNA hybrids, respectively. (a) Robust digestion of 20- and 40-mer ssRNA by 3.64 × 10⁻⁴ nM RNase A. (b) Quantification of the rate of digestion according to the maximum gradient in (a). (c) Robust resection of the RNA strand of the 20- and 40-mer RNA:DNA hybrids by 280 nM RNase H. (d) Quantification of the rate of resection based on the maximum gradient in (c). Error bars represent SEM; n = 3 in all cases; ***p < 0.001.