Figure 3.

Cold exposure recruits newly differentiated ATM2. HFD-fed mice were maintained at 30°C (W: Warm) or exposed to 4°C for 10 days (C: Cold) as indicated in Fig. 1A. (A) Total SVF cell count per fat pad. (B) Frequencies of CD45⁺ cells in live SVF. (C) Frequencies of CD45⁺/F4/80⁺/CD11b⁺/Gr1⁻/Fcer1⁻/siglec-f⁻ total ATM in total SVF. (D) Representative plots for M2/M1 ATM populations. (E) Ratio of the frequencies of ATM2 (CD11c⁻/CD206⁺) over ATM1 (CD11c⁺/CD206⁻). (F) Frequencies of CD45⁻/CD31⁻/PDGFRα⁺/Sca1⁺/CD29⁺ AP in SVF. (G–I) The effects of anti-CSF1R antibody treatment in HFD-fed mice during cold exposure, using the same gating strategy as above. The frequencies of ATM1, ATM2, AP, and BEC in SCAT (G) and VAT (H) in SVF. (I) Cytokine concentrations in VAT lysate. *P < 0.05, **P < 0.01, ***P < 0.001 by Student’s t-test. Data are shown as mean ± SEM.