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. 2019 Jun 20;2:227. doi: 10.1038/s42003-019-0478-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Mcrip1-KO mice die at the neonatal stage due to respiratory failure. a The targeting strategy for Mcrip1-KO mouse generation. Positions of the PCR primers (SU, SUR, and LacZR; for details see methods) that were used for genotyping are indicated. b PCR analysis of genomic DNA isolated from Mcrip1 wild-type (+/+), heterozygous (+/−), or homozygous (−/−) KO mice. c Immunoblot analysis of MCRIP1 expression in MEFs isolated from Mcrip1^+/+ or Mcrip1^−/− mice. β-actin, loading control. d Genotype distribution of mice from a pool of 156 recorded pups that were still alive at P7. The bar graph indicates the observed number of pups; the dashed line indicates the expected number of pups according to Mendel’s law. e Gross morphology of Mcrip1^+/+ and Mcrip1^−/− newborn mice. f Hematoxylin and eosin staining of lungs from Mcrip1^+/+ or Mcrip1^−/− mice at P0. The areas in the small squares in the upper panels were enlarged and are shown below. g Immunohistochemical analysis of MCRIP1 expression in Mcrip1^+/+ and Mcrip1^−/− lungs. Lung tissues isolated from the neonates were sectioned and stained with an anti-MCRIP1 antibody. Arrows indicate the MCRIP1-expressing epithelial layer in Mcrip1^+/+ lungs. f, g Scale bars, 100 μm (upper), 50 μm (lower)