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. 2019 Jun 20;2:227. doi: 10.1038/s42003-019-0478-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Surfactant proteins are down-regulated in Mcrip1-KO mouse lungs. a, b Immunoblotting a and qRT-PCR b analyses of SP-B and SP-C expression in whole-lung lysates isolated from Mcrip1^+/+ and Mcrip1^−/− mice. Data in b represent the mean ± SEM. obtained from three mice. c Immunohistochemical analyses of SP-B and SP-C expression in the lung tissues of Mcrip1^+/+ and Mcrip1^−/− neonates. Scale bars, 100 μm. d–f Immunoblot d, e and qRT-PCR f analyses of the expression of Muc1 and Abca3 in whole-lung lysates isolated from Mcrip1^+/+ and Mcrip1^−/− mice. Data in f represent the mean ± SEM obtained from three mice. NS, not significant. g, h Ultrastructure of AEC2, lamellar bodies, and tubular myelin in Mcrip1^+/+ and Mcrip1^−/− lungs at E21 g and at P0 h. h The areas in the small squares in the middle sets of panels were enlarged and are shown on the right. Arrows indicate lamellar bodies and arrowheads indicate tubular myelin. Scale bars, 2 μm in g. Scale bars, 2 and 1 μm in left and middle panels of h, respectively