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. 2019 Jun 20;2:227. doi: 10.1038/s42003-019-0478-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — MCRIP1 is expressed in the lung epithelium during lung development. a–d Immunohistochemical analysis of the expression of MCRIP1 a, Foxp1 and Foxp2 b, CtBP1 and CtBP2 c, and SP-B and SP-C d in Mcrip1^+/+ lungs at the embryonic stage of E18 and E21. Scale bars, 100 and 50 μm in left and right panels of a, respectively. Scale bar, 50 μm in b–d. e Western blot analysis of whole-lung lysates isolated from Mcrip1^+/+ and Mcrip1^−/− mice. The levels of the indicated proteins were analyzed by immunoblotting with their specific antibodies. β-actin, loading control. f Immunohistochemical analysis of Foxp1 and Foxp2 in lung tissues isolated from Mcrip1^+/+ and Mcrip1^−/− mice. Scale bars, 20 μm