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. 2019 Jun 20;9:8876. doi: 10.1038/s41598-019-45184-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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RT-PCR analysis of differentially expressed sugarcane gene isoforms. Alternative splicing in selected sugarcane genes was analyzed using RT-PCR. Molecular size (bp) of the isoforms is shown using a DNA ladder. Putative alternative transcripts/isoforms are labeled numerically. −: negative control/no template; *: primer-dimer. The top and bottom panels were cropped and grouped from different parts of the same gel for clarity. An uncropped image used to prepare this figure is presented in Supplementary Fig. S3.