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. 2019 Jun 20;9:8929. doi: 10.1038/s41598-019-45263-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Chd1 and Chl1 deletions affect cohesin activities. (A) Strains yIO-120 (wild-type), yAB-2010 (chd1Δ), yAB-2028 (chl1Δ) and yAB-2029 (chd1Δ chl1Δ) were grown to mid-log phase, arrested at the G2/M phase of the cell cycle and processed for cohesion assay (n = 3, P < 0.01). (B) Strains, yAM-895 (tof1Δ) and yAM-903(chd1Δ tof1Δ) were grown to mid-log phase, arrested at the G2/M phase of the cell cycle and processed for cohesion assay (n = 3, P < 0.01). (C) Strains yIO230 (Scc3-6HA), yIO-2046 (chd1Δ Scc3-6HA), yAB-2045 (chl1Δ Scc3-6HA) and yAB-2048 (chd1Δchl1Δ Scc3-6HA) were grown and arrested at the G2/M phase as in A. Chromosome spreads were prepared and stained with DAPI (DNA) and anti-HA (Scc3); (n = 3, P < 0.01). (D) Quantitation of anti-HA (Mcd1) from B. The intensity of Mcd1 was normalized by the intensity of DAPI.