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. 2019 Jun 20;9:8929. doi: 10.1038/s41598-019-45263-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CHD1 and CHL1 are involved in the cell response to genotoxic reagents but not in intra-chromosomal recombination. (A) Strains yIO-001 (wild-type), yAB-2003 (chd1Δ), yAB-2018 (chl1Δ) and yAB-2019 (chd1Δ chl1Δ) were grown to saturation, serially diluted and plated on YPD plates with or without the indicated genotoxic drugs. (B) Strains yIO-163 (RDN1::ADE2), yIO-164 (sir2∆ RDN1::ADE2), yAB-2039 (chd1∆ RDN1::ADE2), yAB-2044 (chl1∆ RDN1::ADE2) and yAB-2043 (chd1∆ chl1∆ RDN1::ADE2) were grown to saturation and plated on YPD plates. The frequency of intra-chromosomal recombination was measured by the percentage of white/red (ADE2/ade2) cells (n = 3).