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. 2019 Jun 20;9:8929. doi: 10.1038/s41598-019-45263-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Deletion of CHD1 and CHL1 affects PDS5 expression. (A) Strains yOG-3006 (wild-type), yAB-2014 (chd1Δ), yAB-2023 (chl1Δ) and yAB-2024 (chd1Δ chl1Δ) were grown to the mid-log phase, lysed. Equal volumes of extract were loaded and analyzed by SDS-PAGE followed by Western blot against Pds5, Mcd1 and Tubulin. The relative signal of Pds5 (B) and Mcd1 (C) was detected by using ImageJ (n = 3, P < 0.05).