Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jun 20;9:8997. doi: 10.1038/s41598-019-45384-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Study of cell culture growth conditions through infiltration into scaffolds. Depth imaging to examine cells (MKN74) penetration into 3D scaffold by indicated three methods as mentioned in the subheading of ‘Cells seeding and hybrid scaffold development’ under Methods section at depth of 0–4 mm. RFU of scaffold penetration into Method 1, MKN74 cells were pipetted onto scaffolds and immediately gelatinized; Method 2, scaffold were soaked in MKN74 cells for 10 min and gelatinized; Method 3, scaffold soaked in MKN74 cells for 10 min, transferred onto 24-well plate, incubated for 30 min and gelatinized. Finally, method 3 was selected based on the highest fluorescence intensity for subsequent studies.