Figure 2.

Inhibitory effect of omeprazole on recombinant giardial triosephosphate isomerase (GlTIM) enzymes lacking the most superficial Cys. After incubation of the enzymes with increasing concentrations of OMP for 2 (A) and 24 (B) h at 37 °C, an aliquot was withdrawn, and the residual activity was monitored as reported in Materials and Methods. The data are the mean of at least three independent experiments. The activity registered for enzyme incubated without omeprazole was considered to represent 100% enzyme activity (2,160, 3,928, and 956 μmol min⁻¹ mg⁻¹ for GlTIM WT, Dmut, and Tmut, respectively).