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. 2019 Jun 20;9:8922. doi: 10.1038/s41598-019-45529-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Omeprazole induces formation of PAS-positive cytoplasmic granules in Giardia trophozoites. Giardia trophozoites at log growth without OMP (A), exposed to 100 μM OMP for 6 h (B), and 500 μM OMP for 1 h (C) were subjected to PAS staining. Insets show the ultrastructural counterparts in each figure panel and highlight the glycogen aggregates. Trophozoites treated with OMP as in (C) were incubated with salivary amylase and then subjected to PAS staining (D). White arrows show PAS-positive granules and their ultrastructural counterparts. Red asterisks show drug-induced ultrastructural damage.