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. 2019 Jun 20;9:8900. doi: 10.1038/s41598-019-45394-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Transverse section of poplar differentiating xylem stained using the Mäule reaction. Top, debarking position; arrow head, boundary of annual ring; bar = 200 µm. (B) Fresh and dry weight of tangential sections prepared from blocks of poplar wood. Region P, phloem; C, cambium and cell expansion zone; X1, xylem formed in the present year; X2, xylem formed in the previous year. (C,D) Syringyl and guaiacyl monomer (C) and p-hydroxyphenyl monomer (D) yield by thioacidolysis. (E) Distribution of H/(G + S) ratio in the radial direction of poplar determined by thioacidolysis. Data are the mean ± SD (B, n = 6; C–E, n = 3).