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. 2019 Jun 20;9:8900. doi: 10.1038/s41598-019-45394-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Time course of p-glucocoumaryl alcohol uptake in hybrid poplar membrane vesicles. Membrane vesicles were incubated with 50 μM p-glucocoumaryl alcohol in the presence (●) or absence (△) of 5 mM Mg/ATP. (B) Negative control for p-glucocoumaryl alcohol uptake. Membrane vesicles of hybrid poplar were incubated with 50 μM p-glucocoumaryl alcohol for 20 min either with 5 mM Mg/ATP, with 5 mM Mg/ADP (+ADP), with 5 mM Mg/AMP (+AMP), or without Mg/ATP (−ATP). “Boiled” refers to a microsomal fraction heat-denatured for 10 min. (C) p-Glucocoumaryl alcohol uptake shows saturation kinetics. Membrane fractions of hybrid poplar were incubated for 5 min in the presence of 5 mM Mg/ATP and each concentration of p-glucocoumaryl alcohol. Inset shows Hanes–Woolf plots. Data are the mean ± SD of three replicates.