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. 2019 Jun 20;9:8865. doi: 10.1038/s41598-019-45087-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MicroRNA (miRNA) expression levels in 40-week-old male chicken testis. (a) Hierarchical cluster analysis of miRNA expression levels. Heat map representation of miRNAs that differed significantly between plasma treatment (P) and control (C) groups. Rows represent transcriptional units. miRNAs that share a similar trend of ascending or descending property are clustered. The yellow represents the maximum Z-score; the blue represents the minimum Z-score. (b) Scatter plot of miRNA expression levels. Red dots represent significant miRNAs; grey dots represent no significant miRNAs. (c) Bar plot of significant miRNAs. FC, fold change. miRNA with |FC| ≥ 1.5 and a p-value < 0.05 is significantly up- or down-regulated. The detailed information of significant miRNAs see Supplementary Table S1.