Fig. 3.

HBx interacts with UCP and forms a ternary complex with UCP and pVHL. a We transfected or not HLK3 cells with 5 μg of GST (glutathione S-transferase)-HBx or GST expression vector, incubated them for 24 h, and performed GST pull-down or immunoprecipitation (IP) assays as indicated. b We mixed GST-UCP (1 μg), F-HBx (0, 0.5, 2 μg, −, +, ++), GST-CDC34 (1 μg), and/or GST (1 μg) proteins, and performed GST pull-down or IP assays as indicated. c We transfected 293T cells with GST-UCP, HA-VHL, F-HBx, and/or GST expression vector (10 μg each), incubated them for 24 h, and performed GST pull-down and IP assays as indicated. d We mixed GST-UCP (1 μg), His-pVHL (1 μg), F-HBx (0.5, 2 μg, +, ++), and/or GST (1 μg) proteins, incubated them at 4 °C for 1 h, and performed pull-down assays as indicated. We repeated the experiments of a–d twice; representative data are shown