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. 2019 Jun 20;9:8993. doi: 10.1038/s41598-019-45299-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Renal epoxy fatty acids and metabolites in lupus prone NZB/W F1 and healthy C57BL/6NCRL mice. (A) Comparison of the cumulative renal EET (5,6-; 8,9-; 11,12-; 14,15-EET), EpOME (9,10-; 12,13-EpOME), DHET (5,6-; 8,9-; 11,12-; 14,15-DHET), DiHOME (9,10-; 12,13-DiHOME) and 20-HETE levels between healthy C57BL/6NCRL (n = 7), prenephritic (n = 6) and nephritic NZB/W F1 mice (n = 5). (B) Renal sEH activity in healthy C57BL/6NCRL (n = 12), prenephritic (n = 7) and nephritic NZB/W F1 mice (n = 7). Bars, median; boxplots, median and interquartile range; whiskers, range; white boxplot, healthy C57BL/6NCRL mice; light grey boxplot, prenephritic NZB/W F1 mice; grey boxplot, nephritic NZB/W F1 mice; *p < 0.05; **p < 0.01; ***p < 0.001. Values that passed the Kolmogorov-Smirnov test for Gaussian distribution were compared using the unpaired t test; otherwise, the Mann-Whitney test was applied.