Fig. 4.

The effect of acute BRT exposure on H₂DCFDA fluorescence following stimulation with A) 20 μM TRAP-6 or B) 20 μM antimycin. TRAP-6 and antimycin treatment used to examine physiological and mitochondrial ROS generation respectively. Platelets were identified by characteristic forward and side scatter parameters with a minimum of 20 000 platelet gated events captured. Data reported as fold change of H₂DCFDA MFI over an unstimulated sample from the same treatment condition. Vehicle is 0.1% (v/v) DMSO HEPES Saline. Data are mean ± SD. n = 6 Statistical significance compared to DMSO vehicle. *p < 0.05, **p < 0.01.