Figure 5.

ThT Decreases the Wet-heat Resistance Level of Endospores

(A) Diagram describing the wet-heat resistance assay shown in panel (B). Cells were committed to sporulation (culture for 3.5 h in RM), then moved to RM with and without ThT. Spores were treated at 80°C for 30 min. See Methods for details.

(B) Survivability of endospores relative to spores produced without ThT. Wet-heat survivability is lower when spores are formed in the presence of 10 μM ThT. Survivability is comparable when ThT is added to the endospores already prepared in RM without ThT. Error bars are 95% confidence intervals for Poisson distribution based on the number of spores observed. Data from three technical replica and three independent biological replicates.