Comparison of miRNA levels in the kidneys of pregnant and non-pregnant mice. A total of 887 miRNAs were detected in the kidneys of pregnant and non-pregnant mice. Of the 887 mature miRNAs detected, 163 miRNAs were found to have potential differential expression (≥ ± 2-fold change, outside dotted line); 128 upregulated (red), 35 downregulated (blue), and 724 miRNAs remained unchanged in pregnancy (black). Plot was generated using commercially available data analysis software (QIAGEN, Data analysis centre). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)