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. 2019 Jun 15;6:556–563. doi: 10.1016/j.toxrep.2019.06.005

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© 2019 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 6 — Fucoidan induced apoptosis in HepG2 cancer cells (constituted with different concentration of fucoidan for 48 h and staining with annexin-V FITC/PI. The values are presented as mean ± SD in triplicate and significance (p ≤0.05) determined by student t-test between fucoidan treated vs untreated control. A. Each quadrant indicates the percentage of normal cells (annexin V-/PI-), early apoptosis (annexin V+/PI-) and late apoptosis (annexin V+/PI+). B & C. Fucoidan (200 μg/mL) altered nuclear morphology by Hoechst 33,342 stains and mitochondrial membrane potential (ΔΨm) by JC-1stains were observed under fluorescence microscopy (original magnification, 400x). Red fluorescence indicates mitochondria with intact membrane potential and green fluorescence indicates de-energized mitochondria).