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. 2016 Nov 26;33(5):351–359. doi: 10.5511/plantbiotechnology.16.0926a

Figure 1. Phylogenetic tree of ISAs based on deduced amino acid sequences and sequence alignment of ISAs in four conserved regions. (A) Phylogenetic tree of ISAs based on deduced amino acid sequences. The tree was constructed using the neighbor-joining method (Saitou and Nei 1987). Scale bar represents evolutionary distance. Bootstrap values were calculated from 1,000 replications. The deduced amino acid sequences of sweet potato IbISAs protein (in box) were derived in this study (IbISA1, accession LC052789; IbISA2, accession LC052790; IbISA3, accession LC052791). Accession numbers for genes encoding ISAs of other plant spices are: Arabidopsis thaliana AtISA1, AEC09752; AtISA2, AEE27558; AtISA3, AEE82713; potato (Solanum tuberosum) StISA1, AAN15317; StISA2, AAN15318; StISA3, AAN15319; maize (Zea mays) ZmISA1, EU970890; ZmISA2, AAO17048; ZmISA3, AAO17049; rice (Oryza sativa) OsISA1, BAC75533; OsISA2, AAT93894; OsISA3, XP_450961; pea (Pisum sativum) PsISA1, AAZ81835; PsISA2, AAZ81836; PsISA3, AAZ81837; common bean (Phaseolus vulgaris) PvISA1, BAF52941; PvISA2, BAF52942; PvISA3, BAF52943; sweet potato (Ipomoea batatas) IbISA1, AAY84833; wheat (Triticum aestivum) TaISA3, AEV92948; cassava (Manihot esculenta) MaISA3, ADD10143. (B) Multiple sequence alignment of ISAs in four conserved regions. Amino acid sequences in Regions I to IV are highly conserved in the α-amylase family. In particular, eight amino acid residues (shaded dark gray) are highly conserved in all active members of the α-amylases, ISA1 and ISA3. Region IV of ISA2s shows replaced residues from H (His) to N (Asn).

Figure 1. Phylogenetic tree of ISAs based on deduced amino acid sequences and sequence alignment of ISAs in four conserved regions. (A) Phylogenetic tree of ISAs based on deduced amino acid sequences. The tree was constructed using the neighbor-joining method (Saitou and Nei 1987). Scale bar represents evolutionary distance. Bootstrap values were calculated from 1,000 replications. The deduced amino acid sequences of sweet potato IbISAs protein (in box) were derived in this study (IbISA1, accession LC052789; IbISA2, accession LC052790; IbISA3, accession LC052791). Accession numbers for genes encoding ISAs of other plant spices are: Arabidopsis thaliana AtISA1, AEC09752; AtISA2, AEE27558; AtISA3, AEE82713; potato (Solanum tuberosum) StISA1, AAN15317; StISA2, AAN15318; StISA3, AAN15319; maize (Zea mays) ZmISA1, EU970890; ZmISA2, AAO17048; ZmISA3, AAO17049; rice (Oryza sativa) OsISA1, BAC75533; OsISA2, AAT93894; OsISA3, XP_450961; pea (Pisum sativum) PsISA1, AAZ81835; PsISA2, AAZ81836; PsISA3, AAZ81837; common bean (Phaseolus vulgaris) PvISA1, BAF52941; PvISA2, BAF52942; PvISA3, BAF52943; sweet potato (Ipomoea batatas) IbISA1, AAY84833; wheat (Triticum aestivum) TaISA3, AEV92948; cassava (Manihot esculenta) MaISA3, ADD10143. (B) Multiple sequence alignment of ISAs in four conserved regions. Amino acid sequences in Regions I to IV are highly conserved in the α-amylase family. In particular, eight amino acid residues (shaded dark gray) are highly conserved in all active members of the α-amylases, ISA1 and ISA3. Region IV of ISA2s shows replaced residues from H (His) to N (Asn).