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. 2019 Jun 14;10:1365. doi: 10.3389/fimmu.2019.01365

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Copyright © 2019 Ahmad, Hanif, Xubiao, Lulu, Shahid, Dayong and Rijun.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Tricine-SDS-PAGE and analysis of recombinant peptide, (A) Tricine-SDS-PAGE of the cell culture media from P.pastoris expressing secreted LL-37Tα1. Lane M, molecular weight markers; Lane C, control (supernatant of X33/PpICZαA); Lane 1 and 2 (supernatant X33/PpICZαA-LL-37Tα1) peptide expression after methanol (144 h) induction and arrow in the lane indicated 3.9 kDa polypeptide. (B) Tricine-SDS-PAGE of recombinant purified LL-37Tα1. Lane M, molecular weight markers; Lane C, control (X33/PpICZαA); Lane 1, a sample of the purified recombinant peptide and arrow indicated LL-37Tα1 (3.9 kDa) expression. (C) The elution pattern of RP-HPLC C18 column of the purified recombinant LL-37Tα1 and the high peak indicates fraction that contains LL-37Tα1. (D) ESI-MS analysis of purified recombinant LL-37Tα1 peptide.