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Cytokine expression of CD4+ T cells stimulated with Sf2a or S. Typhimurium LPS ex vivo at 7 days after primary immunization. Bar graphs depicted the proportions of antigen-specific cells expressing IFNγ (A) and IL-4 (B), which were calculated by deducting the percentages of cytokine-positive cells of unstimulated samples from the antigen-stimulated values of corresponding samples. Data were summarized from two (n=6) independent experiments. * P < 0.05, ** P <0.01, *** P <0.001.
