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Systemic and mucosal antibody concentrations against Sf2a and S. Typhimurium LPS. Serum IgG, IgG2a, IgG1and mucosal IgA against Sf2a LPS (A-D) and S. Typhimurium LPS (E-H) were measured by ELISA at 14 days after secondary immunization. The RDAS strains (Vac1 and Vac2) could stimulate comparable levels of Sf2a LPS-specific antibody responses with the constitutive antigen synthesis strains (Vac3 and Vac4). * P < 0.05, ** P <0.01, *** P <0.001.
