Figure 6.

Radiosensitization by mitochondrial transplantation in vitro. (A) Experimental schematic to test radiosensitizing effect of mitochondrial transplantation in U87 cells. (B) Expression levels of key proteins in mitochondrial apoptotic pathway were measured by western blot. (C) Apoptosis was quantified by combined staining of annexin V and PI, and fluorescence was analyzed using flow cytometry. (D) Cell index values were determined every 15 min using a real-time cell electronic sensing (RT-CES) system for up to 106 h. (E) Effects of mitochondrial transplantation followed by X-ray irradiation on clonogenic potential of U87 cells. (F) Statistical analysis of colony formation assay. *p<0.05, **p<0.01 vs. control group; ^#p<0.05 vs. 4 Gy irradiation alone. (G) Statistical analysis of the sensitizer enhancement ratios at 10% survival level (SER₁₀).