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. 2019 Jun 2;9(12):3659–3673. doi: 10.7150/thno.32126

Figure 5.

Figure 5

RIP3 deletion enhances the immunogenicity of the tumor microenvironment during CAC. (A) Colon tissues from adenoma-bearing mice were stained with an anti-F4/80 antibody. Representative images and quantitative data are shown. Original magnification, ×200. (B-H) The fractions of peri-tumoral CD3+ T cells (B) and CD8+ T cells (C), the expression of IL-10 (D), PD-1 (E), CD44 (F) and CD107a (G) on CD8+ T cells, and the fraction of peri-tumoral CD19+ B cells (H) were determined by flow cytometry. SSC, side scatter. (I, J, K, L, and N) The fractions of peri-tumoral Gr1+ CD11b+ MDSC (I), F4/80- CD11c+ MHC II+ dendritic cells (J), and CD11c- Gr1- CD11b+ F4/80+ TAMs (K), and the expression of CD206 (L) and PD-L1 (N) in TAMs were assessed by flow cytometry. (M) Lysates of distal colons from CAC-bearing mice were prepared, and Arg1 mRNA expression was analyzed using qRT-PCR. Data are means ± SEM (n = 5), *p < 0.05, **p < 0.01, ***p < 0.001. Flow cytometry experiments were carried out twice.