Figure 8.
MC granule-primed hUCB-MSCs ameliorate excisional wound in the mice more efficiently than naïve and histamine-treated cells. The dorsal skin of BALB/c mice was punched with a 5-mm-diameter biopsy punch, and 1 × 106 MC-primed hUCB-MSCs were immediately infused subcutaneously. Each group of mice was monitored and sacrificed on day 14 for further ex vivo examinations. (A) Representative gross images of the wound area in the dorsal region and quantification of the wound size at each indicated time. (B) Detailed histogram for the quantification of the wound size on day 14. (C) Immunohistochemistry of CD31 expression in the dorsal skin of the mice (n=5), bar = 100 μm. Wound: Excisional wound mice group, MSC: hUCB-MSC-injected group, MC-MSC: MC-primed hUCB-MSC-injected group, Hist-MSC: histamine-treated hUCB-MSC-injected group. The results are shown as the mean ± SD.