Table 1.

Analyzed parameters and time points

	Study days1	Parameters
Body weight	1, 2, 4, 5, 11–14, 16, 17, 19, 26, 33, 36, 38, 43
Blood chemistry	−6, 16, 24, 30, 37, 44, 50	creatinine, urea nitrogen, phosphorus, calcium, sodium, potassium, AST, ALT, AP, CK, LDH, GLDH, Fe
Hematology	−6, 12, 26, 39	hematocrit, erythrocytes, hemoglobin, reticulocytes, leukocytes, thrombocytes, MCH, MCHC, MCV
Urine	−6, 16, 30, 37, 44	protein, creatinine, phosphorus, calcium, sodium, potassium, volume, osmolality
GFR	−8, 18, 46	GFR
Necropsy	49, 50	gross pathology and histopathology

Parameters were assessed before, during, and after AD feeding (days 0–20) in control (n = 10), AD-2K (n = 10), and AD-1K (n = 16) rats; values of days –8 and –6 were used as baseline. AD, adenine at 0.75% in the diet; AD-1K rats, rats with unilateral nephrectomy, adenine at 0.75% in the diet; AD-2K rats, sham-operated rats, adenine at 0.75% in the diet; GFR, glomerular filtration rate.

¹Samples were collected on 1–2 consecutive days and collated.