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. 2019 Jun 14;7:107. doi: 10.3389/fcell.2019.00107

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Copyright © 2019 Pijuan, Barceló, Moreno, Maiques, Sisó, Marti, Macià and Panosa.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Overview of the wound healing assay preparation protocols. (A) Step-by-step scheme showing the differences between wound healing protocol using a culture insert (option A) and using pipette tip (option B). Phase-contrast microscopy shows gap appearance and both cell fronts just before to start the time-lapse experiment. (B) Measurements of wound width (μm) in culture insert (n = 50) or pipette tip (n = 50). Mean values (thick horizontal lines), confidence limits (α = 0.05, thin horizontal lines), and coefficients of variation (label) are shown.