Table 2.
Properties of the 30 cellulase candidates from the GH5 family used to determine their cellulolytic activity
| ORFsa ID | Sample ID | Amino acid length | Best BLAST hit vs. NCBI (enzyme annotation) | Accession number | Identity % | Signal peptideb |
|---|---|---|---|---|---|---|
| c589026_g1_1 | Cel5A-h1 | 344 | Glycoside hydrolase | WP_022932641.1 | 70 | N |
| c607167_g3_1 | Cel5A-h2 | 556 | Glycoside hydrolase | WP_020965515.1 | 49 | Y |
| c350756_g1_5 | Cel5A-h10 | 744 | Glycoside hydrolase family 5 protein | WP_097035831.1 | 66 | Y |
| c1499489_g1_2 | Cel5A-h11 | 317 | Glycoside hydrolase family 5 protein | WP_031559633.1 | 68 | N |
| c676361_g1_1 | Cel5A-h12 | 721 | T9SS C-terminal target domain-containing protein | WP_101480019.1 | 52 | Y |
| c423733_g1_1 | Cel5A-h13 | 303 | Glycoside hydrolase family 5 protein | WP_013498507.1 | 99 | N |
| c7388_g1_1 | Cel5A-h14 | 499 | Endoglucanase | WP_115153074.1 | 57 | N |
| c430069_g1_3 | Cel5A-h15 | 512 | Endoglucanase | CDC18601.1 | 52 | N |
| c592373_g1_1 | Cel5A-h16 | 633 | Glycoside hydrolase family 5 protein | WP_084156318.1 | 55 | Y |
| c581150_g3_2 | Cel5A-h17 | 602 | Putative carbohydrate-active enzyme | ADX05709.1 | 64 | N |
| c602169_g11_1 | Cel5A-h21 | 437 | Cellulase | ADU86908.1 | 68 | N |
| c535046_g1_1 | Cel5A-h23 | 410 | Glycoside hydrolase family 5 protein | WP_013499238.1 | 99 | Y |
| c613169_g10_1 | Cel5A-h24 | 335 | Endoglucanase | WP_103869183.1 | 65 | N |
| c660653_g1_1 | Cel5A-h26 | 304 | Hypothetical protein RASY3_16565 | EXM37921.1 | 95 | N |
| c602981_g3_1 | Cel5A-h27 | 698 | Glycoside hydrolase family 5 protein | WP_072812340.1 | 92 | Y |
| c630880_g1_1 | Cel5A-h28 | 480 | Endoglucanase | CBL34359.1 | 50 | Y |
| c533175_g1_1 | Cel5A-h31 | 415 | Cellulase | AHF24954.1 | 87 | Y |
| c591423_g1_2 | Cel5A-h32 | 609 | Multispecies: hypothetical protein | WP_072418845.1 | 44 | Y |
| c569332_g1_1 | Cel5A-h35 | 617 | Multispecies: glycosyl hydrolase family 5 | WP_031534432.1 | 48 | Y |
| c606942_g4_1 | Cel5A-h37 | 731 | Endoglucanase | WP_028517111.1 | 48 | Y |
| c608068_g7_1 | Cel5A-h38 | 343 | Hypothetical protein | WP_044916478.1 | 68 | N |
| c597031_g2_1 | Cel5A-h41 | 445 | Glycoside hydrolase family 5 protein | OUM69972.1 | 55 | N |
| c573287_g1_1 | Cel5A-h42 | 807 | Endoglucanase | CDE12421.1 | 45 | Y |
| c605251_g1_1 | Cel5A-h44 | 387 | Glycoside hydrolase family 5 protein | WP_014775421.1 | 38 | N |
| c607602_g6_1 | Cel5A-h45 | 593 | Cellulose 1,4-beta-cellobiosidase | WP_037304596.1 | 99 | N |
| c483261_g1_1 | Cel5A-h47 | 365 | Glycoside hydrolase family 5 protein | WP_080550482.1 | 72 | N |
| c607437_g11_1 | Cel5A-h49 | 577 | Glycosyl hydrolase family 5 | WP_013497304.1 | 99 | N |
| c607437_g9_1 | Cel5A-h50 | 690 | Glycoside hydrolase family 5 | WP_100068403.1 | 83 | Y |
| c586094_g1_3 | Cel5A-h53 | 736 | T9SS C-terminal target domain-containing protein | WP_101480019.1 | 60 | Y |
| c541302_g1_1 | Cel5A-h54 | 433 | Glycoside hydrolase family 5 protein | WP_083379902.1 | 65 | Y |
aORFs, open reading frames
bPresence (Y) of a signal peptide was predicted using the SignalP program