Table 2.

Selected genes of lipid metabolism in the genome of B. raffinosifermentans LS3

Gene identifier	Previous function/similarity [reference yeast]a	New function prediction	Amino acid % identity (coverage)	Process
ARAD1B07414	Succinyl CoA ligase (alpha subunit) [sc]	ATP:citrate lyase (subunit) EC:2.3.3.8	88% (99%) with YALI0E34793	Formation of cytosolic acetyl-CoA, precursor of FA synthesis
ARAD1D32164	Succinyl CoA ligase (beta subunit) [sc]	ATP:citrate lyase (subunit) EC:2.3.3.8	82% (100%) with YALI0D24431	Formation of cytosolic acetyl-CoA, precursor of FA synthesis
ARAD1C08250	Acyl-CoA:diacylglycerol acyltransferase (DGAT) [sc]	DGAT (no change) EC:2.3.1.20	69% (59%) with YALI0E32769	Formation of TG
ARAD1D42460	Acyl-CoA:sterol acyltransferase [sc]	DGAT EC:2.3.1.20	42% (86%) with YALI0D07986	Formation of TG
ARAD1C30118	Weakly similar to FAT2 peroxisomal AMP binding protein [sc]	Acyl-CoA synthetase	38% (99%) with YALI0E12419 (10 paralogs). PTS1 motifb	Formation of acyl-CoA (peroxisomal activation of FA)

^aIn the current (first) version of the annotated genome of LS3 in GRYC database. First-round annotation was performed using BLAST against S. cerevisiae (sc) as the reference yeast to look for similarity

^bAKL in C terminus of the protein which likely targets it to peroxisome