Figure 2.
The impact of CORM-A1 and DETA/NO on protein levels in lung tissue in the experimental models of metastasis of 4T1-luc2-tdTomato cells. (A) Total VASP, (B) pVASPSer239, (F) PKG-1α and (G) HO-1 densitometric analysis (mean optical density of bands of protein tested to β-actin) and representative blots (D), (E), (H), and (I) respectively. (C) Calculated pVASPSer239/VASP ratio. “L” means lower dose of CORM-A1 and DETA/NO (0.5 mg/kg/12h and 1.57 mg/kg/24h, respectively; treatment schedule indicated by yellow circle in Scheme 1A and Figure 1A). “H” means higher dose of CORM-A1 and DETA/NO (1.5 mg/kg/12h and 2.358 mg/kg/24h, respectively; treatment schedule indicated by green circle in Scheme 1A and Figure 1A). Data presented as mean with standard deviation with points for individual measurements. Number of mice: 4-5 per group. Healthy mice used as a control: 5. Statistical analysis: Dunnett's multiple comparison tests. *p< 0.05 as compared to control or as indicated.