Figure 6.
CORM-A1 and DETA/NO combination therapy effect on total and phosphorylated VASP protein level in aorta from mice bearing 4T1 orthotopic tumors. (A) Total VASP, (B) pVASPSer239, (F) PKG-1α and (G) HO-1 densitometric analysis (mean optical density of bands of protein tested to β-actin) and representative blots (D), (E), (H), and (I) respectively. (C) Calculated pVASPSer239/VASP ratio. Mice were inoculated with 4T1 cells into fat pad and 7 days later, when tumors were palpable, the i.p. administration of CORM-A1 (0.5 mg/kg, every 12 h) and DETA/NO (1.570 mg/kg, every 24h) started. Tissues collected on day 28 of experiment. Data are presented as mean ±SD with individual measurements depicted. Number of samples analyzed: 3 per group. Statistical analysis: Dunnett's multiple comparison tests. *p< 0.05 as compared to control or as indicated.