Figure 6.

ALKBH5 expression is negatively correlated with CYR61 expression in RM disease. (A) CYR61 mRNA levels in first-trimester human villous tissues from RM patients (n = 65) or in HCs (n = 57) were determined by qRT-PCR. (B) Levels of CYR61 expression in first-trimester human villous tissues from RM patients or in HCs were determined by western blotting. (C) Representative immunofluorescence images of ALKBH5 and CYR61 in frozen first-trimester villous sections obtained from RM patients and HCs. Fluorescence signals specific to anti-ALKBH5 antibodies appear green; anti-CYR61 antibodies appear red; and the DAPI-stained nuclei appear blue. (D) Global RNA m⁶A methylation level correlated positively with CYR61 mRNA levels in the villous tissue of RM patients and HCs. (E) ALKBH5 mRNA level correlated negatively with the CYR61 mRNA level in the villous tissues of RM patients and HCs.