Figure 2.

The effect of berberine (BBR), rosuvastatin, combination of BBR and rosuvastatin on galectin‐3 expression on THP‐1‐derived macrophages. THP‐1‐derived macrophages were pretreated with phosphate‐buffered saline (PBS), BBR (25 μM), rosuvastatin (25 μM), and BBR (25 μM) and rosuvastatin (25 μM) for 1 hr and then stimulated by 100‐μg/ml oxidized low‐density lipoprotein (ox‐LDL). (a) Western blots show the protein expression of galectin‐3 at 24 hr after stimulation by ox‐LDL. (b) Relative gene expression of galectin‐3 in THP‐1‐derived macrophages was determined by real‐time PCR at 12 hr after stimulation by ox‐LDL. (c) Laser scanning confocal microscopy was used to confirm galectin‐3 expression on the cell membrane of THP‐1‐derived macrophages in different groups. Data are represented as mean ± SD. n ≥ 3. ^* p < 0.05, ^** p < 0.01 versus ox‐LDL group; #p < 0.05 versus PBS group. Scale bar = 100 μm [Colour figure can be viewed at wileyonlinelibrary.com]