Figure 5.

Activin‐A inhibits osteoclastogenesis in CD14+ monocultures. CD14+ cells isolated from a buffy coat from a healthy donor were cultured for 14 and 21 days in the presence of M‐CSF and RANK‐L (a) without, and (b) with 50 ng/ml Activin‐A. The number of TRAcP positive multinucleated cells (more than three nuclei) were counted (c). n = 3 (Wilcoxon matched‐pairs signed rank test, ^* p ≤ 0.05). Activin‐A significantly inhibits osteoclast formation. M‐CSF: macrophage colony‐stimulating factor; RANK‐L: receptor activator of nuclear factor kappa‐B ligand; TRAcP: tartrate resistant acid phosphatase [Color figure can be viewed at wileyonlinelibrary.com]