Table 1.
Primers used for quantitative PCR
| Gene | Sequence 5′‐3′ | Amplicon length (bp) |
Ensemble Gene ID |
|---|---|---|---|
| HPRT1 | TgACCTTgATTTATTTTgCATACC | 101 | ENSG00000165704 |
| CgAgCAAgACgTTCAgTCCT | |||
| PBGD | TgCAgTTTgAAATCATTgCTATgTC | 84 | ENSG00000113721 |
| AACAggCTTTTCTCTCCAATCTTAgA | |||
| CSF1 | CCgAggAggTgTCggAgTAC | 100 | ENSG00000184371 |
| AATTTggCACgAggTCTCCAT | |||
| CTCggAgCTCTgATgTgTTgAA | |||
| DCSTAMP | ATTTTCTCAgTgAgCAAgCAgTTTC | 101 | ENSG0000016493 |
| AgAATCATggATAATATCTTgAgTTCCTT | |||
| ACVR1 | CAgCTgCCCACTAAAggAAAAT | 68 | ENSG00000115170 |
| AATAATgAggCCAACCTCCAAgT | |||
| FKBP1A/FKBP12 | gATCCgAggCTgggAAgAAg | 68 | ENSG00000088832 |
| ggAgATATAgTCAgTTTggCTCTCTgA | |||
| ID‐1 | ACgTgCTgCTCTACgACATgA | 56 | ENSG00000125968 |
| TgggCACCAgCTCCTTgA |
Note. ACVR1: Activin A receptor type I; CFS1: colony‐stimulating factor1 (coding for macrophage‐colony stimulating factor [M‐CSF]); DC‐STAMP: dendritic cell‐specific transmembrane protein; FKBP1A: FK506 binding protein 1A (coding for FK binding protein 12 [FKBP12]); HPRT1: hypoxanthine phosphoribosyltransferase 1; ID‐1: Inhibitor of DNA binding 1 protein; PBGD: porphobilinogen deaminase ; PCR: polymerase chain reaction.
For each gene, the first oligonucleotide sequence represents the forward primer, the second sequence represents the reverse primer.