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. 2018 Nov 4;35(1):e2732. doi: 10.1002/btpr.2732

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© 2018 The Authors. Biotechnology Progress published by Wiley Periodicals, Inc. on behalf of American Institute of Chemical Engineers.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Fragmentation during purification of Fab A. (A) The initial purification process flow for Fab A. (B) Time‐dependent fragmentation of Fab A visualized with HP‐SEC profiles during incubation at pH 3.4. (C) HCP and fragment levels (after pH 3.5 incubation) in purification intermediates. (D) Fragmentation (after pH 3.5 incubation) visualized with HP‐SEC profiles for Fractogel® SO₃(M) capture product, Fractogel® SO₃(M) product further purified with CH1 affinity chromatography, and Fractogel® SO₃(M) product further purified with CH1 affinity chromatography and spiked with CH1 unbound fraction.