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. 2018 Nov 4;35(1):e2732. doi: 10.1002/btpr.2732

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© 2018 The Authors. Biotechnology Progress published by Wiley Periodicals, Inc. on behalf of American Institute of Chemical Engineers.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Confirmation of Cathepsin L as the root cause of the fragmentation. (A) Fragmentation visualized by HP‐SEC profiles (during pH 3.4 incubation) for Fab A cleaved by recombinant murine Cathepsin L or CHO Cathepsin L (in CH1 unbound fraction). Fab A concentration was 2.5 mg/mL and 1 mM DTT was added in the sample mixture for the recombinant murine Cathepsin L. (B) Intact mass spectroscopy ion chromatograms for Fab A fragments generated by recombinant mouse Cathepsin L or CHO Cathepsin L (in CH1 unbound fraction).