Figure 5.

Chromatographic behavior of Cathepsin L in bind‐and‐elute chromatography. (A) Fragmentation (during pH 3.4 incubation) of Fab A visualized by HP‐SEC profiles after purification from CCF by cation exchange, affinity and mixed mode chromatography. (B) Elution profiles of Fab A and Cathepsin L on different cation exchanger resins eluted in a 0–500 mM NaCl, 20 CV linear gradient at pH 5.0. (C) Elution profiles of Fab A and Cathepsin L on Fractogel® SO₃(M) in a 0–500 mM NaCl, 20 CV linear gradient at different pH values. (D) The effects of washes on elution product protease level and step yield observed during Fab A purification with Fractogel® SO₃(M). The elution product was acidified to pH 3.4 and incubated at 37°C for 3 h and fragment level was measured by HP‐SEC.